Tiziana Rossetti

Principal

2019

Senior Analyst (Healthcare fund), Investments in Early Stage Start-ups

2017 - 2019

Business Analyst (Venture Capital)

2017 - 2017

Venture Capital Analyst. Early stage business analysis (startups), due diligence, market and competition analysis

CEO and Founder

2015 - 2017

Postdoctoral Researcher

2015 - 2015

> Summary I worked at the Hertie Institute of Tübingen for six months, during which I did histological studies in mouse tissues. In Professor S. Di Giovanni lab I worked on the characterization of stem cells in adult mice spine cord. >Molecular and Cell Biology techniques used: Genotyping (DNA extraction and PCR analysis), processing of the tissue (perfusion, cryopreservation) and sectioning (cryostat), Immunohistology, microscopy. > Responsibility: To design and perform experiments, to perform data analysis and present the results during weekly meetings with colleagues. To write scientific manuscripts. Achieved objectives: Histological Characterization of stem cells into spinal cord of transgenic mice.

Research Associate

2013 - 2015

> Summary I worked at the MRC, CSC, Imperial College from 'August 2013 until March 2015. During the nearly two years that I worked with Professor E. Petretto we discovered two gene networks each about a thousand elements and associated with neurological disorders. The project concluded with two recent publications in the Nature Journal, which are the result of a collaboration between several academic institutes and the pharmaceutical company UCB >Molecular and Cell Biology techniques used: Cell culture (in primary rat neurons, 293T, Helas, BV2, 3T3 fibroblasts, mHippo 3), production, titratione and application of lentiviral vectors, western blot, gene expression analysis with qPCR, production of libraries for sequencing, genotyping by PCR, Immunohistology and Immunocytochemistry, oxidative stress assay, FACS. > Responsibility as Lab Manager: To order reagents, management of the laboratory, management of lab equipment (i.e. maintenance and support to new users), assistance to PhD students, management of the organic material (i.e. stocking and shipments to collaborators), management of colonies of transgenic rats (management of the Home Office records, termination, genotyping etc.) > Responsibility as Researcher to design and perform experiments, to perform data analysis and present the results during meetings with colleges and collaborators. To Write proposals for research grants (and scientific manuscripts. Achievements: -I managed the molecular biology laboratory - I managed the transgenic animal colonies -I produced a research grant manuscript to request funding to MRC -I validated two different networks of genes associated with neurological disorders (Epilepsy and Bipolar Disorder)

Research associate

2012 - 2013

> Summary I worked in Professor M. Modo’s group at the McGowan Institute for regenerative medicine (University of Pittsburgh) from April 2012 until June 2013; the laboratory focus on development of new strategies for tissue engineering (brain repair, stroke therapies) . My protocol ensures up to 80% of viable cells in solutions containing up to 250k of cells per microliter and for a period equal to eight hours after harvesting. >Molecular and Cell Biology techniques used: cell cultures (Stroc05), western blot, survival assays, Immunohistology and Immunocitochemistry, microsurgery (Middle Cerebral Artery Occlusion) and analysis of ischemic injury through behavioral tests. > Responsibility as lab manager: To order reagents, management of the laboratory, management of lab equipment (i.e. maintenance and support to new users), management of the rat colony (maintaining up to date the IACUC records, termination of the animals according to the approved animal license etc.), management of the strictly regulated psychotropic substances. > Responsibility as researcher: To design and perform experiments, to perform data analysis and present the results during internal meetings, scientific retreat (McGowan Institute for regenerative medicine, UPMC, retreat) and international conferences. Drafting the scientific manuscript Rossetti et all, 2015 and two license for laboratory animal studies approved by the IACUC (the Institutional Animal Care and Use Committee , approved in 2012). Achievements: -I developed an SOP for the preparation of suspensions of human stem cells for clinical applications -I wrote a scientific manuscript published on a peer review journal (Intracerebral Cell Implantation: Preparation and Characterization of Cell Suspensions. Rossetti T, F Nicholls, M. Cell Transplant mode. 2015 Dec 30.) -I wrote two animal licenses, both approved in 2012 by the IACUC -I managed the laboratory and warehouse

PhD student

2008 - 2012

> Summary I worked at Royal Holloway University of London from October 2008 to March 2012. During this time I investigated the role of neurogenesis in the SVZ in the post-ischemic spontaneous recovery. I also collaborated in a study that explored the effects of retroviral infection in endothelial cells of the porcine carotid. >Molecular and Cell Biology techniques used: Cell cultures (293T, Hela, 3T3 fibroblasts), production, titration of lentiviral vectors, western blot, analysis of the viral titer by FACS or qPCR, Immunohistology and Immunocytochemistry, analysis of cellular cycle by FACS, analysis of cell proliferation by MTT assay, ELISA, microsurgery (MCAO, intracranial injections), molecular cloning. > Responsibility as student-researcher: To design and perform experiments, to perform data analysis and present the results during weekly meetings with colleagues and during the annual conferences (internal and external of the University ). To write scientific manuscripts and theses, to assist graduate students during laboratory practice and their final research project. Achievements: -I proved that the post-ischemic recovery in murine brain is reduced if the Sub Ventricular Zone neurogenesis is abolished (pilot study). -I wrote and published my thesis manuscript: "The Role of Neurogenesis in Post-ischemic spontaneous recovery". https://pure.royalholloway.ac.uk/portal/en/persons/tiziana-rossetti(8edd0e89-05cb-4b9a-b51c-a5c1e5948200).html -I produced several lots of Lentiviral Vectors. -My Lentiviral Vectors were used on a project on endothelial cells derived from porcine carotid artery. (Transgene delivery to endothelial cultures derived from ex vivo porcine carotid artery. J Andoh at al. Stroke Transl Res. 2013 Oct; 4 (5): 50. -I worked as assistant professor for three years (supporting undergrads during lectures) -I supervised undergrads for their final project (one student every year for a total of three students)

Postgraduate student

2006 - 2008

Full management of the project: 1. The effect of 5-HT1A R expression in ventral CA1 Significant contribution to the project: 2. Sporadic autonomic dysregulation and death associated with excessive serotoninergic autoinhibition. Position: postgraduate student, EMBL Monterotondo, Supervisors: Dr. Cornelius Gross, Senior Scientist EMBL Monterotondo Techniques: Animal care and breeding, genotyping by PCR and analysis of phenotype by autoradiography and behavioral tests (fear conditioning, open field, tail suspension test, elevated plus maze), pharmacologic treatments